Ingingo yasohotse mu Itumanaho ry'itangazamakuru. Ingingo ya metero ebyiri za DNA zishyizwe muri karnel ntoya ya selile yumuntu kubera ko chromatin, urusobe rwa ADNATIN, amplex na poroteyine, bihindura imiterere yoroheje, ariko imiterere yoroshye, ariko igoye. Kwiga uburyo bwo gupakira ADN, abahanga kwisi yose bikoreshwa nukwitwa hamwe kubahirika kwa chromosomes (3c), kandi kimwe mubitanga umusaruro muribyo ni uburyo bwa hi-c. Iragufasha kumenya imibonano ya ADN ya geno yose ukoresheje ibice byinshi.
Muri ibyo, ariko, hari ikibazo: Imirimo ya Hi-C isaba micrograms nyinshi za ADN - ni ukuvuga ingirabuzimafatizo zifite umuryango wihariye wa Chromatina. Aya makuru agomba guhindagurika kugirango abone ishusho isanzwe itazirikana ibiranga ADN mumaseli yindege.
Nkuko "umuntu usanzwe" adahari, uburyo gakondo bwa hi-c ntishobora kwerekana, niyihe mumikoranire myinshi yo kubice bya ADN bibaho icyarimwe muri selile imwe. Byongeye kandi, iyi "portraire cortrait" ntisobanura kumva uburyo inzira yumubiri iganisha kumiterere yimiterere itatu ya chromatin.
Ati: "Turabona inzego zimwe, nk'urugero, yiswe Topologs ifitanye isano na TOPOLOLOSS (Tadada), mu makarita ya ADN yagereranije, ariko ntituzi niba babaho mu tugari twihariye, cyangwa ibi ni ibihangano bitandukanye. Byongeye kandi, tuzi ko duhereye ku mvugo ya gene, ibintu bitandukanye bibaho no mu tugari twa tissue imwe - Kuva hano hari ikibazo gisanzwe cyuko urwego rutandukanye, " Umwanditsi wa Mikhail Gelowand, Visi Perezida Slatitha akurikije ubushakashatsi busanzwe.
Gukemura ibyo bibazo no gukora ubushakashatsi bwa Hi-C bikwiranye n'ingirabuzimafatizo ku giti cyabo, abashakashatsi b'ibigo byinshi bateje imbere uburyo bwitwa Hi-C Ingirabuzimafatizo. Itsinda rya Skoltech riyobowe na Profeseri wa Sholteand na Porofeseri w'ikigo cya Centre ya Cather, Catherine Itorero ryo Gutegura Umutekano wa Hi-C kandi usuzume imitungo y'ibanze ya Drosophila.
Abakozi ba bagenzi babo bo muri ikigo cya biologiya cya Biologiya y'Ishuri Rikuru ry'Uburusiya na kaminuza ya Leta ya Moscou yitiriwe ubushakashatsi bwakozwe mu kigo cy'Uburusiya-Igifaransa cyitiriwe ingirabuzimafatizo zifite ingirabuzimafatizo zifite drosophila .
Amakipe yatangiye nintambwe zisanzwe zuburyo bwa Hi-C, aho imiterere ya Chromatine ikosowe na chimique, kandi ADN yaraciwe "kugirango" ibice bikunze kuba hafi, byagaragaye "kudoda ". Ariko rero, aho gukoresha ADN yose icyarimwe, abahanga bokoroje * ntoya ya ADN muri buri selire bakoresheje Polymeraoge Polymerase. Iyi Polymeyese ikoreshwa mugihe cyongerewe ADN, igice, kubera ubushobozi bwayo bwo gukora urugero runini rwa ADN ndetse no kurugero ruto cyane hamwe numubare muto muto kurenza izindi polymerases.
Ariko, byaje kugaragara ko aya mahirwe ya ADN yoroshye, nubwo ari ukuri kwikoporora, birashobora "gusimbuka" hagati ya ADN Molekile, igira ubumwe bwa ARNILECOME ko algorithm ya HA-C idashobora gutandukanywa n'imikoranire nyayo. Abashakashatsi rero bagombaga kuzana uburyo bwo kwangwa kuri ibyo bidasanzwe "gusimbuka" kwa Polymeyese.
Bakoresheje uburyo bwabo bushya kuri selile ya drosophila kugirango bamenye niba ibinyabuzima bitandukanye bifite amahame mboneragihugu yibanze kubipakira Chromatin. Ubushakashatsi bwambere kuri selile yinyamabere bwerekanye ko habaho tade zijyanye namakarita yo gutumanaho yabonetse nabaturage Hi-c, ariko ntabwo ari mu tugari twihariye. Ariko, kwiga ingirabuzimafatizo bya Drosophila byerekanaga ko aba domeni bari muri buri selire runaka.
Kugira ngo wumve uburyo bwo guhuza ibinyabuzima bishinzwe gushinga indangarugero irambye, ubushakashatsi bwinyongera buzakenerwa; Mugihe abahanga batanze icyitegererezo cya bibiri. Umwe muri bo avuga ko Chromatin muri Drosophila yateguwe na Methoran Mechanism ya "gukomera", ni ukuvuga bimwe mu bice byayo birashoboka ko bihuzwa hagati yabo. Nk'uko ikindi, gusobanura icyiswe uburyo bwitwa Shangesm Orrucsm, ibigo binini bya poroteyine bitera imigozi kuva ku nsanganyamatsiko ya ADN no kubera iyi paki.
Ati: "Ahari kimwe mubibazo bishimishije ni ukumenya niba amategeko yo kuzinga chromatin ari kimwe muburyo butandukanye bwibinyabuzima. Twakoresheje uburyo bwa hi-c of selile imwe, Drozophile, twasanze domaine isa na domaine muri selile za nyammaliya nayo irahari muri kanone. Ariko, izo nzego ziteganijwe cyane kuruta mu mayira y'inyamabere. "Nyuma ya Schosltech yarangije umunyeshuri, umwe mu banditsi ba mbere b'ikinyamakuru.
Ati: "Tuzakomeza kwiga ubwubatsi bwa chromatina nuburyo bwo gushiraho imirongo na tadov. Haracyari ibibazo byinshi muriki gice nta gisubizo. Tumaze kumenya ko ubwo buryo mubinyabuzima bimwe bishobora gutandukana, ariko ubwihindurize bwa chromatin bugenda buzenguruka muri rusange? Niba dushaka kubyumva kurwego ruhagije, dukeneye kuzuza icyuho, twiga imiterere ya Chromatin mubinyabuzima bidasanzwe, ntabwo ari iperereza gusa. Kubwibyo, dusanzwe dukorana na sponges marine, umusemburo na amiebas, "
Ku bwe, itsinda naryo risezeranya impinduka zishoboka mu miterere ya Chromatina n'indwara, iterambere ry'umubiri no gusaza. Tehraeva agira ati: "Niba dukeka ko ubwubatsi bwa Chromatina bufitanye isano rya bugufi n'imvugo ya genes, noneho, dusubiza ibibazo, tuzashobora guhangana n'amabwiriza yo guteza imbere umubiri w'umuntu, gusaza n'indwara."
Inzobere mu Kigo cya Biologiya zo mu Geni y'Ikirusiya cyanditswe mu Bumenyi bw'Uburusiya, kaminuza ya Leta ya Moscou yitiriwe ubushakashatsi mu bumenyi bw'ubufaransa, Ikigo cy'igihugu gishinzwe ubushakashatsi ku Bufaransa, Ikigo cy'imiryango y'Ikirusiya-Igifaransa gifite uruhare mu bushakashatsi.
Inkomoko: Ubumenyi bwambaye ubusa